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Addgene inc pcfj601
Pcfj601, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mos1+transposase/pCFJ601+-+Peft-3+Mos1+transposase+(Plasmid+%2334874)/pm41673492-246-52-55
Average 93 stars, based on 39 article reviews
pcfj601 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Injection:

Article Title: Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1
Article Snippet: .. Injection mixes contained 50 ng/μl MosSCI-targeting vector, 50 ng/μl eft-3p :: Mos1 transposase (pCFJ601, Addgene #34874), 10 ng/μl rab-3p :: mCherry (pGH8, Addgene #19359), 2.5 ng/μl myo-2p :: mCherry (pCFJ90, Addgene #19327), 5 ng/μl myo-3p :: mCherry (pCFJ104, Addgene #19328), and 10 ng/μl hsp-16 . .. 1 :: peel-1 negative selection (pMA122, Addgene #34873) .

Article Title: Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1
Article Snippet: .. Injection mixes contained 50 ng/μl MosSCI-targeting vector, 50 ng/μl eft-3p :: Mos1 transposase (pCFJ601, Addgene #34874), 10 ng/μl rab-3p :: mCherry (pGH8, Addgene #19359), 2.5 ng/μl myo-2p :: mCherry (pCFJ90, Addgene #19327), 5 ng/μl myo-3p :: mCherry (pCFJ104, Addgene #19328), and 10 ng/μl hsp-16 . .. 1 :: peel-1 negative selection (pMA122, Addgene #34873) ( ).

Article Title: Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci
Article Snippet: .. Injection mixes contained 50 ng/μl MosSCI-targeting vector, 50 ng/μl eft-3p :: Mos1 transposase (pCFJ601, Addgene #34874), 10 ng/μl rab-3p :: mCherry (pGH8, Addgene #19359), 2.5 ng/μl myo-2p :: mCherry (pCFJ90, Addgene #19327), 5 ng/μl myo-3p :: mCherry (pCFJ104, Addgene #19328), and 10 ng/μl hsp-16 . .. 1 :: peel-1 negative selection (pMA122, Addgene #34873).

Generated:

Article Title: A high-content imaging approach to profile C. elegans embryonic development
Article Snippet: The two transgene modules encoding the yellow muscle nuclei, P hlh-1::mCherry::his-72 and P hlh-1::GFP::his-72 , were cloned in tandem into pCFJ178 (Addgene plasmid #19331 ) for insertion at cxTi10882 on chromosome IV. .. Single-copy transgenes were generated by injecting a mixture of the above transgene constructs, a plasmid encoding the Mos1 transposase [pCFJ601, P eft-3::Mos1 transposase (Addgene plasmid #34874 ), 50 ng/μl] and three plasmids encoding fluorescent markers for negative selection [pCFJ90, P myo-2::mCherry (Addgene plasmid #19327 ), 2.5 ng/μl; pCFJ104, P myo-3::mCherry (Addgene plasmid #19328 ), 5 ng/μl; and pGH8, P rab-3::mCherry (Addgene plasmid #19359 ), 10 ng/μl] into strains EG6429 (outcrossed from EG4322; ttTi5605 , chromosome II) or EG6250 ( cxTi10882 , chromosome IV). ..

Construct:

Article Title: A high-content imaging approach to profile C. elegans embryonic development
Article Snippet: The two transgene modules encoding the yellow muscle nuclei, P hlh-1::mCherry::his-72 and P hlh-1::GFP::his-72 , were cloned in tandem into pCFJ178 (Addgene plasmid #19331 ) for insertion at cxTi10882 on chromosome IV. .. Single-copy transgenes were generated by injecting a mixture of the above transgene constructs, a plasmid encoding the Mos1 transposase [pCFJ601, P eft-3::Mos1 transposase (Addgene plasmid #34874 ), 50 ng/μl] and three plasmids encoding fluorescent markers for negative selection [pCFJ90, P myo-2::mCherry (Addgene plasmid #19327 ), 2.5 ng/μl; pCFJ104, P myo-3::mCherry (Addgene plasmid #19328 ), 5 ng/μl; and pGH8, P rab-3::mCherry (Addgene plasmid #19359 ), 10 ng/μl] into strains EG6429 (outcrossed from EG4322; ttTi5605 , chromosome II) or EG6250 ( cxTi10882 , chromosome IV). ..

Plasmid Preparation:

Article Title: A high-content imaging approach to profile C. elegans embryonic development
Article Snippet: The two transgene modules encoding the yellow muscle nuclei, P hlh-1::mCherry::his-72 and P hlh-1::GFP::his-72 , were cloned in tandem into pCFJ178 (Addgene plasmid #19331 ) for insertion at cxTi10882 on chromosome IV. .. Single-copy transgenes were generated by injecting a mixture of the above transgene constructs, a plasmid encoding the Mos1 transposase [pCFJ601, P eft-3::Mos1 transposase (Addgene plasmid #34874 ), 50 ng/μl] and three plasmids encoding fluorescent markers for negative selection [pCFJ90, P myo-2::mCherry (Addgene plasmid #19327 ), 2.5 ng/μl; pCFJ104, P myo-3::mCherry (Addgene plasmid #19328 ), 5 ng/μl; and pGH8, P rab-3::mCherry (Addgene plasmid #19359 ), 10 ng/μl] into strains EG6429 (outcrossed from EG4322; ttTi5605 , chromosome II) or EG6250 ( cxTi10882 , chromosome IV). ..

Article Title: Effects of telomerase overexpression in the model organism Caenorhabditis elegans.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Selection:

Article Title: A high-content imaging approach to profile C. elegans embryonic development
Article Snippet: The two transgene modules encoding the yellow muscle nuclei, P hlh-1::mCherry::his-72 and P hlh-1::GFP::his-72 , were cloned in tandem into pCFJ178 (Addgene plasmid #19331 ) for insertion at cxTi10882 on chromosome IV. .. Single-copy transgenes were generated by injecting a mixture of the above transgene constructs, a plasmid encoding the Mos1 transposase [pCFJ601, P eft-3::Mos1 transposase (Addgene plasmid #34874 ), 50 ng/μl] and three plasmids encoding fluorescent markers for negative selection [pCFJ90, P myo-2::mCherry (Addgene plasmid #19327 ), 2.5 ng/μl; pCFJ104, P myo-3::mCherry (Addgene plasmid #19328 ), 5 ng/μl; and pGH8, P rab-3::mCherry (Addgene plasmid #19359 ), 10 ng/μl] into strains EG6429 (outcrossed from EG4322; ttTi5605 , chromosome II) or EG6250 ( cxTi10882 , chromosome IV). ..



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